Table 1 Primer sequences used for amplification of the 16S V1-V3 and V3-V4 region
From: An accurate and efficient experimental approach for characterization of the complex oral microbiota
16S region | Name of primer | Primer sequence |
|---|---|---|
V1–V3 | Illumina_16S_27F | 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGAGTTTGATCMTGGCTCAG |
Illumina_16S_534R | 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATTACCGCGGCTGCTGG | |
V3–V4 | Illumina_16S_341F | 5′ -TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG |
Illumina_16S_805R | 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC |