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Fig. 4 | Microbiome

Fig. 4

From: An accurate and efficient experimental approach for characterization of the complex oral microbiota

Fig. 4

Procrustes analyses demonstrates significant correlation between oral bacterial composition obtained with the V1–V3 and V3–V4 regions. Procrustes analysis of the bacterial composition of V1–V3 (red) and V3–V4 (blue) regions was calculated using the unweighted UniFrac metric. β-diversity distances were computed at the genus level using the a closed-reference OTU picking method against the Greengenes or b HOMD database, and c the de novo OTU picking with uchime or d ChimeraSlayer chimera removal. For a given sample, red lines connect to 16S sequence data from the V1–V3 region while blue lines connect to points generated from the V3–V4 sequence data. The M 2 fit reported is from a Procrustes transformation over the first two principal coordinates, while the P value is calculated from an empirically determined distribution of M 2 values over 10,000 Monte Carlo simulations.

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