Fig. 3
From: An accurate and efficient experimental approach for characterization of the complex oral microbiota
Boxplots of Pielou’s index J for V1–V3 and V3–V4 OTUs support a negative relationship between observed oral sample species richness and evenness. Pielou’s index J was estimated independently for all generated taxa summaries at the species level to evaluate species evenness detected by the V1–V3 and V3–V4 regions. Analysis was based on OTUs picked based on the a closed-reference OTU picking method against the Greengenes or b HOMD database, and c the de novo OTU picking with uchime or d ChimeraSlayer chimera removal. The x-axis shows the value for Pielou’s index J, and the y-axis presents the amplicon regions tested. J takes values between 0 and 1, with values closer to 1 representing more even quantities of the different species within a community