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Fig. 1 | Microbiome

Fig. 1

From: Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs)

Fig. 1

Impact of MBRA cultivation on microbial diversity. Microbial diversity of triplicate MBRA communities inoculated with one of three donor fecal samples (Donor A, blue circles; Donor B, green circles; Donor C, purple circles) or in six replicate MBRA communities inoculated with an equal mass of all three donor fecal samples (Pool, black circles) was determined by sequencing the V4 region of the 16S rRNA gene from samples collected daily over 21 days in culture. Microbial diversity (Inverse Simpson, a), total number of OTUs (b), and evenness of OTU distribution (Simpson Evenness, c) was calculated from OTUs (≥97 % average nucleotide identity (ANI)) that were randomly subsampled to 10,000 sequences over 100 iterations. The mean value for the replicate reactors as a function of time in culture is plotted (day 0 = fecal inoculum; error bars represent standard deviation of the mean)

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Microbiome

ISSN: 2049-2618

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