Fig. 2

Flowchart of the OTU-picking pipeline. 16S rRNA gene primers specific for the Blautia genus were identified with pprospector, and the primer pair was used to generate 16S rRNA gene amplicons. Enriched PCR samples were targeted for SMRT-seq to get long CCS sequencing reads. CCS reads were taxonomically classified with QIIME pipeline, and reads classified to the genus Blautia were kept. Genetic distances were computed using pairwise alignments. Hierarchical clustering of the resulting dissimilarity matrix was performed at sequence similarities 1 to 6 % with 1 % increments. The final set of OTUs was filtered by requiring at least 10 or more supporting reads