Skip to main content
Fig. 9 | Microbiome

Fig. 9

From: Humanized microbiota mice as a model of recurrent Clostridium difficile disease

Fig. 9

HMbMouse model of recurrent CDI. Mice were administered a five-antibiotic cocktail in drinking water ad libitum for 4 days followed by an IP injection of clindamycin (10 mg kg−1). Twenty-four hours after IP injection, mice were challenged with the clinical NAP1/B1/027 ribotype strain, CD3017 (a: n = 5, b: n = 4, c: n = 4). Relapse was induced by further IP injection of clindamycin (10 mg kg−1). Shaded area and error bars indicate standard deviation from mean. Star = clindamycin-induced relapse. ND spores non-detectable. C. difficile Spore numbers were enumerated by homogenizing fecal samples in 500 μl PBS and heating to 65 °C for 30 min to kill vegetative cells followed by serial dilution and plating in replicate on TCCFA plates

Back to article page
\