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Fig. 5 | Microbiome

Fig. 5

From: Humanized microbiota mice as a model of recurrent Clostridium difficile disease

Fig. 5

Experiment design. A five-antibiotic cocktail consisting of kanamycin (0.4 mg ml−1), gentamicin (0.035 mg ml−1), colistin (850 U ml−1), metronidazole (0.215 mg ml−1), and vancomycin (0.045 mg ml−1)) was administered ad libitum in drinking water for 3, 4, or 5 days. Antibiotic water was changed to sterile antibiotic free water and 24 h later mice were administered an intra-peritoneal injection of clindamycin (10 mg kg−1). Twenty-four hours post clindamycin IP, mice are challenged with C. difficile spores by oral gavage and monitored for signs of disease. Recurrent CDI is induced by administration of a further clindamycin injection (10 mg kg−1)

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