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Table 1 Correlation and detection limit of mass seq read counts with spore counts

From: Amplicon sequencing for the quantification of spoilage microbiota in complex foods including bacterial spores

OTUs Spore spike strain y = ax + b Regression coefficient (R 2) Det. limit x = (yb)/a (10log(sp/sample) at y = 1 read Spores/sample
OTU 6 G. stearothermophilus ATCC7953 y = 0.644x − 0.5384 0.9617 0.8 7.0E+00
OTU 3 G. thermoglucosidans TNO-09.020 y = 0.7464x − 0.3915 0.9778 0.5 3.0E+00
OTU 5 G. thermoglucosidans TNO-09.020 y = 0.7222x − 0.5543 0.9348 0.8 6.0E+00
OTU 4 B. sporothermodurans IC4 y = 0.9669x − 1.9271 0.9978 2.0 9.8E+01
OTU 2 B. cereus TNO-02.0143 y = 0.9239x − 2.5864 0.9655 2.8 6.3E+02
OTU 7 B. subtilis A163 y = 1.095x − 2.9748 0.9449 2.7 5.2E+02
OTU 1 B. subtilis A163 (canned food and spike) y = 0.011x + 3.8187 0.0111 −347.2 0.0E+00
  1. The table shows for a tenfold dilution series of spore-mix batches, the linear regression parameters (a, b, R 2) for 10log(# spores) and 10log(# reads) plotted on X- and Y-axis, respectively. R 2 indicates the quality of linearity while x at y = 1 yields the detection limit (10log(#spores) for one detected mass sequence read. Calculations are performed for each of the detected known OTUs representing the five different species in the spore-mix. Note that OTU 3 and OTU 5 both represent strain TNO-09.020