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Table 4 Xander assembly of pooled rhizosphere samples

From: Xander: employing a novel method for efficient gene-targeted metagenomic assembly

Gene nirK nifH rplB
Crop C M S C M S C M S
# chimeric clusters removed 16 207 11 0 1 0 14 28 44
# protein contig clustersa 1993 1807 1581 39 57 41 19,287 20,463 17,334
# OTUs at 95 % aa identity 741 674 582 14 24 17 6100 6887 6004
Median (aa) 215 230 208 294 256 255 274 274 274
Longest (aa) 380 372 370 296 296 296 285 285 284
Median % aa identity 88.3 84.7 87.8 92.7 91.9 91.6 77.7 75.8 76.3
Max % aa identity 100 99.4 98.6 100 100 100 100 100 100
# reads covering kmers 27,404 19,815 16,661 411 534 461 225,985 179,867 149,661
Relative abundance 0.121 0.11 0.111 0.002 0.003 0.003 1 1 1
  1. C corn, M Miscanthus, S switchgrass, nirK nitrite reductase gene, nifH nitrogenase reductase gene, rplB ribosomal protein L2 gene
  2. a# protein contig clusters: number of protein contigs clustered at 99 % aa identity