Xander: employing a novel method for efficient gene-targeted metagenomic assembly

Microbiome

Table 4 Xander assembly of pooled rhizosphere samples

Gene	nirK			nifH			rplB
Crop	C	M	S	C	M	S	C	M	S
# chimeric clusters removed	16	207	11	0	1	0	14	28	44
# protein contig clusters^a	1993	1807	1581	39	57	41	19,287	20,463	17,334
# OTUs at 95 % aa identity	741	674	582	14	24	17	6100	6887	6004
Median (aa)	215	230	208	294	256	255	274	274	274
Longest (aa)	380	372	370	296	296	296	285	285	284
Median % aa identity	88.3	84.7	87.8	92.7	91.9	91.6	77.7	75.8	76.3
Max % aa identity	100	99.4	98.6	100	100	100	100	100	100
# reads covering kmers	27,404	19,815	16,661	411	534	461	225,985	179,867	149,661
Relative abundance	0.121	0.11	0.111	0.002	0.003	0.003	1	1	1

C corn, M Miscanthus, S switchgrass, nirK nitrite reductase gene, nifH nitrogenase reductase gene, rplB ribosomal protein L2 gene
^a# protein contig clusters: number of protein contigs clustered at 99 % aa identity

ISSN: 2049-2618