Fig. 3From: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choiceLongitudinal bacterial profile of two babies (pre-weaning), comparing FISH and 16S rRNA gene sequencing data. a, b—sequencing data (27f-Mix primer set); c, d—FISH data. a, c Baby N-BF, natural birth, breast-fed only; b, d Baby C-MF, C-section, one bottle formula/day introduced from 5 weeks. FISH probes used were Eub338 (total bacterial count), Erec482 (Lachnospiraceae), Fprau645 (F. prausnitzii group of the Ruminococcaceae), Bif164 (Bifidobacterium genus), Rum730 (Rfla729 + Rbro730) (Ruminococcus flavefaciens and R. bromii subclusters of the Ruminococcaceae), Prop853 (Veillonellaceae), Bac303 (Bacteroides-Prevotella group), LAB158 (Lactobacillaceae and Enterococcaceae) and EntD (Enterobacteriaceae). The same colouring scheme has been used to illustrate overlap between bacterial taxa identified using the two methodsBack to article page