Skip to main content
Fig. 3 | Microbiome

Fig. 3

From: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice

Fig. 3

Longitudinal bacterial profile of two babies (pre-weaning), comparing FISH and 16S rRNA gene sequencing data. a, b—sequencing data (27f-Mix primer set); c, d—FISH data. a, c Baby N-BF, natural birth, breast-fed only; b, d Baby C-MF, C-section, one bottle formula/day introduced from 5 weeks. FISH probes used were Eub338 (total bacterial count), Erec482 (Lachnospiraceae), Fprau645 (F. prausnitzii group of the Ruminococcaceae), Bif164 (Bifidobacterium genus), Rum730 (Rfla729 + Rbro730) (Ruminococcus flavefaciens and R. bromii subclusters of the Ruminococcaceae), Prop853 (Veillonellaceae), Bac303 (Bacteroides-Prevotella group), LAB158 (Lactobacillaceae and Enterococcaceae) and EntD (Enterobacteriaceae). The same colouring scheme has been used to illustrate overlap between bacterial taxa identified using the two methods

Back to article page