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Fig. 2 | Microbiome

Fig. 2

From: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice

Fig. 2

Comparison of bacterial families detected in faecal samples from two babies. Sequence data is based on 16S rRNA gene amplicons obtained using the 27f-YM (blue) or 27f-Mix (red) forward primers. a Baby N-BF: Data shows the mean percentage of sequences in each bacterial family after 15 separate DNA extractions at seven time points. b Baby C-MF: Data shows the mean percentage of sequences in each bacterial family after six separate DNA extractions at three time points. For both panels, individual data points are plotted as open circles; centre lines in the box plots show the medians; crosses represent sample means; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. Plotted using BoxPlotR [52]

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