Alpha diversity pattern with time and triclosan exposure. Shannon diversity measures are plotted as interquartile range with median for each triclosan exposure class and time point grouping. There is an initial drop in alpha diversity at the beginning of the study. Baseline samples are significantly more diverse than all other time points (denoted by asterisk; Tukey’s HSD, adjusted P ≤ 0.05). Within day 7, square brackets denote that the control samples differ significantly from low exposure (P = 0.026; estimated difference in mean: 0.327; 95% CI: 0.031 to 0.623) and high exposure samples (P = 0.001; estimated difference in mean: 0.464; 95% CI: 0.167 to 0.760) and the solvent samples differ significantly from the high exposure samples (P = 0.017; estimated difference in mean: −0.348; 95% CI: −0.644 to −0.052) For each boxplot, n = 8 except day 14, low (n = 7).