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Figure 5 | Microbiome

Figure 5

From: A straightforward and efficient analytical pipeline for metaproteome characterization

Figure 5

Validation of the pipeline performance and reproducibility by murine fecal metaproteome analysis. (A) Analysis of pipeline reproducibility at the ORF level. Left: scatter plot illustrating the correlation between the number of PSMs detected in the two replicates (r = Pearson correlation coefficient); each point in the plot represents an identified ORF. Right: Venn diagrams depicting the overlap of ORF identifications between the two replicates (left diagram, all identified ORFs; right diagram, ORF identified with at least two PSMs; percentage of overlap in brackets). (B) Analysis of pipeline reproducibility at the peptide level. Left: scatter plot illustrating the correlation between the number of PSMs detected in the two pipeline replicates (r = Pearson correlation coefficient); each point in the plot represents an identified peptide. Right: Venn diagrams depicting the overlap of peptide identifications between the two pipeline replicates (left diagram, all identified peptides; right diagram, peptide identified with at least two PSMs; percentage of overlap in brackets). (C) Pie charts illustrating the percentage distribution of the identified PSMs according to LCA taxonomy (left: replicate 1; right: replicate 2).

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