Table 1 Raw sequence reads and post-QA/QC sequence reads statistics from nine 250PE and three 300PE MiSeq runs
Run type | Pool ID (run ID) | Number of samples per pool | Raw sequence reads | QA/QC sequence reads | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
[DNA] (pM) | Cluster density | %phiX | % ≥Q30 | Reported machine error | Number of read pairs | Total number of reads | Median read length | Mean read length | |||
(K/mm2) | |||||||||||
(Forward; Reverse)a | (S1, S2)a | (S1, S2) ± SDa | |||||||||
250PE | 199–205 (130325) | 352 | 5.5 | 509 | 16.3 | 87.4 | 1.24 | 9,413,692 | 5,994,627; 6,243,014 | 268; 256 | 266.04 ± 7.12; 246.99 ± 23.98 |
250PE | 207–212 (130525) | 387 | 6 | 583 | 14.4 | 86.3 | 1.32 | 10,656,207 | 7,438,345; 7,196,027 | 268; 259 | 265.41}8.02; 248.67}23.14 |
250PE | 217–222 (130612) | 337 | 6.2 | 650 | 15.1 | 84.1 | 1.5 | 11,706,459 | 7,806,262; 8,021,515 | 268; 244 | 265.03}8.48; 240.30}20.49 |
250PE | 223–228 (130705) | 364 | 5.5 | 839 | 10.7 | 84.6 | 1.24 | 13,398,346 | 9,703,996; 9,226,330 | 268; 261 | 265.00}8.84; 250.24}23.38 |
250PE | 229–234 (130708) | 366 | 4 | 625 | 8.4 | 81.1 | 1.57 | 11,071,952 | 8,089,983; 7,167,888 | 268; 232 | 265.85}6.94; 228.97}28.21 |
250PE | 241–246 (130805) | 305 | 12 | 527 | 8.9 | 92.5 | 1.34 | 9,615,279 | 7,393,672; 7,180,867 | 268; 264 | 266.21}6.23; 255.01}19.15 |
250PE | 247–252 (130801) | 299 | 9 | 429 | 17.3 | 91.2 | 1.07 | 7,787,755 | 5,267,816; 5,190,563 | 268; 266 | 266.39}7.73; 256.12}20.82 |
250PE | 259–265 (130813) | 426 | 7 | 445 | 9.3 | 90.7 | 1.46 | 7,919,928 | 5,920,074; 5,425,815 | 268; 265 | 265.10}9.83; 255.54}17.47 |
250PE | 235–240 (130815)b | 371 | 11 | 351 | 14.7 | 90.7 | 1.71 | 8,517,215 | 6,268,044; 5,848,845 | 268; 267 | 267.73}2.75; 262.44}13.29 |
300PE | 235–240 (130916)b | 371 | 11 | 514 | 13.9 | 90.9 | 1.44 | 12,522,115 | 9,328,369 | 499 | 497.19}6.93 |
300PE | 266–270 (131011) | 271 | 11 | 441 | 15.4 | 91.3 | 1.54 | 10,699,820 | 8,111,221 | 485 | 488.03}9.78 |
300PE | 271–275 (131015) | 276 | 12 | 592 | 12.3 | 90.4 | 1.52 | 14,241,937 | 10,912,710 | 495 | 491.05}10.56 |