Figure 4

Diversity of the underlying reads for putative CDS annotated as phospho-N-acetylmuramoyl-pentapeptide transferase. Each variant has a final protein sequence (grey bars). Below are the nucleotide sequences used to reconstruct the protein. The nucleotide sequences are color coded based on season and sampling location (yellow colors = summer; blue colors = winter; gold = GOM13; yellow = GOM12; blue-green = GOM04; blue = GOM06). Unique sequence reads were aligned to the assembled nucleotide sequence using CLUSTAL W. The colors represent SNP locations in relation to the assembled nucleotide sequence (green = A; blue = C; black = G; red = T). The three protein variants were aligned to each other using CLUSTAL W. Colors along the length of the protein represent amino acid changes.