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Table 2 Sequence data and diversity indexes

From: Respiratory tract clinical sample selection for microbiota analysis in patients with pulmonary tuberculosis

Sample type

Characteristics

Sequence type

  

Bacterial (16S)

Fungal (ITS1)

TB

Controls

TB

Controls

Nasal

Total sequencesa

124,977

106,729

76,480

73,858 (4)

Observed OTUs

318

348

378

345

Chao1

708

627

684

585

Shannon (H')

3.0*

4.2*

6.9

6.6

Oropharynx

Total sequencesa

140,987

95,693

29,759

22,376 (3)

Observed OTUs

577

882

98

131

Chao1

1262

1941

153

209

Shannon (H')

4.8

5.8

3.8

4.8

Sputum

Total sequences

120,681

ND

66,278

ND

Observed OTUs

827

ND

102

ND

Chao1

1857

ND

154

ND

Shannon (H')

6.1

ND

3.4

ND

  1. ND not available. aNumber in parenthesis indicates samples for which sequences were obtained, if less than 6. *p < 0.05 (U Mann-Whitney) indicates significance between TB patients and healthy controls for 16S rRNA gene sequences.