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Figure 4 | Microbiome

Figure 4

From: Choice of bacterial DNA extraction method from fecal material influences community structure as evaluated by metagenomic analysis

Figure 4

Hierarchical clustering. (A) Read counts mapped to the 25 most abundant bacterial genera for the three samples with three replicates for the two DNA extraction methods. Overall, the biggest observable difference results from between-sample variability, and regardless of the DNA extraction method used, the bacterial abundance profile can be assigned to the right individual. (B) Read counts mapped to the 25 most abundant bacterial genera for comparison of the effect of homogenizing and scraping of the samples. All the replicates were extracted from the same biological sample with the same DNA extraction method (HMP); therefore, the only source of variation comes from homogenizing or scraping of the samples before DNA extraction. As shown by the branch length in the sample clustering dendrogram, we observe higher between-replicate variability in the case of scraped samples.

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