Figure 2

Summary of read mapping to known reference genome sequences of different taxonomic groups. As expected, the highest numbers of reads could be mapped to known bacterial genomes, with a slightly higher number of reads mapped with the HMP method (P = 0.0212). Despite overall low number of reads mapped to eukaryotic organism reference genomes, the differences in read counts attributed to the DNA extraction methods were highly significant, with the MetaHIT method resulting in a higher number of reads of eukaryotic origin in all cases. P-values were calculated with two-way ANOVA.