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Table 1 Number and types of libraries and reads obtained in the Amazon Continuum Project, June 2010, R/V Knorr

From: The Amazon continuum dataset: quantitative metagenomic and metatranscriptomic inventories of the Amazon River plume, June 2010

 

Metagenomes

Non-selective metatranscriptomes

Poly(A)-selective metatranscriptomes

Data type

Total community DNA

Total community mRNA

Eukaryotic community mRNAa

# Stations sampled

6

6

6

# Size fractions sampled

2

2

1

# Replicates

2

2

2

# Samples

24

24

12

# Raw reads

3.68 × 108

8.12 × 108

4.61 × 108

# Joined reads post QC

9.50 × 107

1.62 × 108

1.01 × 108

Average joined read length (bp)

205

190

185

# rRNA reads

-

9.53 × 107

2.34 × 105

# Potential protein-encoding reads

9.44 × 107

6.52 × 107

9.86 × 107

  1. aThe selective metatranscriptomes captured poly(A)- tailed transcripts and are therefore systematically biased against transcripts from eukaryotic organelles. #, number of; QC, quality control.