CopyRighter flowchart for the correction of microbial amplicon datasets. (A) Pre-computation of genome copy number (GCN) based on a tree-based taxonomy and reference genomes. (B) The processing of microbial data through off-the-shelf programs. (C) The correction of microbial data to estimate relative abundance, absolute abundance and average GCN in given samples. OTU, operational taxonomic unit; qPCR, quantitative polymerase chain reaction; rRNA, ribosomal RNA.