Exploring the butyrate-producing bacterial community based on 16SrRNA gene analysis. (A) Candidates were split intobutyryl-CoA:acetate CoA-transferase (but; grey bars) and butyratekinase (buk; coarse white bars) containing groups. (B)Individual composition of but (Acidaminococcus sp. - olive,Anaerostipes sp. - dark green, Coprococcus sp. - dark red,Eubacterium sp. - black, Faecalibacterium sp. - darkpurple, Megasphaera sp. - light purple,Peptoniphilus sp. - blue, Oscillibacter sp. - grey andRoseburia sp. - orange) and buk (Anaerotruncus -coarse grey, C. beijerinckii - coarse white, C. butyricum -coarse light blue, C. perfringens - coarse light yellow,Enterococcus sp. - light green and Subdoligranulum sp. -coarse light red) communities are given. (C) Quantitative PCR datatargeting the 16S genes of Faecalibacterium sp. (purple bar),Roseburia sp./E. rectale (orange bar) and C.butyricum (white coarse bar). Note: Coprococcus sp. isconsidered to contain both but and buk genes. The error barsrepresent the range on duplicate measurements. All results are corrected formultiple 16S rRNA copy numbers of individual bacteria (see Methods). ?- Butyrate production was shown for one strain of Subdoligranulum sp.and it is unclear whether all members of this genus have the ability tosynthesize butyrate.