Quantitative PCR analysis of butyryl-CoA:acetate CoA-transferase( but ) and butyrate kinase( buk ) genes. But ofAcidaminococcus sp. (light green bars), F. prausnitzii(purple bars) and Roseburia sp. /E. rectale (orange bars) aswell as buk linked to C. butyricum, C. acetobutylicum andC. perfringens (white coarse bars) were targeted. Percentage wascalculated based on total 16S rRNA gene qPCR data. Results weremultiplied by five to account for multiple 16S rRNA gene copy numbersof intestinal bacteria. The error bars represent the range on duplicatemeasurements.