Analysis of obtained butyrate kinase ( buk ) sequences.(A) Neighbor joining tree of all buk reference sequences(closest hit from FrameBot) matching our amplicon data. Amount of ampliconsequences per closest match and reference sequence GI number (GenBank) areprovided. Sequences marked with * or + highlight known butyrateproducers [5, 29].Pie charts illustrate the origin of amplicon sequences (red: patient 200,green: patient 206, blue: patient 207, yellow: patient 210 and grey: healthycontrols). Note: relative abundance was investigated and the proportion of eachcolor in the pie charts does not correspond to actual abundance of genes insamples. Relative community patterns per individual sample are presented inAdditional file 1: Figure S6. Bootstrap values areindicated. (B) Conservation analysis of reference sequences from PanelA (dashed line) and of obtained amplicon sequences (thick grey line).The displayed sequence on top corresponds to the consensus sequence of allreference sequences. Bold amino acids demonstrate conserved sites (>95%) inboth reference and amplicon sequences. Blue amino acids on both sequence endsillustrate primer binding sites.