Analysis of obtained butyryl-CoA:acetate CoA-transferase( but ) sequences. (A) Neighbor joining tree ofall but reference sequences (closest hit from FrameBot) matching ouramplicon data. Amount of amplicon sequences per closest match and referencesequence GI number (GenBank) are provided. Sequences marked with * are derivedfrom bacteria with known but activity [5, 11]. Pie charts illustrate theorigin of amplicon sequences (red: patient 200, green: patient 206, blue:patient 207, yellow: patient 210 and grey: healthy controls). Note: relativeabundance was investigated and the proportion of each color in the pie chartsdoes not correspond to actual abundance of genes in samples. Relative communitypatterns per individual sample are presented in Additional file 1: Figure S5. Bootstrap values are indicated. (B)Conservation analysis of reference sequences from Panel A (dashed line)and of obtained amplicon sequences (thick grey line). The displayed sequence ontop corresponds to the consensus sequence of all reference sequences. Boldamino acids demonstrate conserved sites (>95;%) in both reference and ampliconsequences. Blue amino acids on both the sequence ends illustrate primer bindingsites.