Bubble charts showing 16S profile reproducibility at human oral taxon (HOT) designation level (98.5% sequence similarity) in replicate biofilm growth wells. Batches 2 and 3 were grown at different time points and batch 2 was also subjected to an additional centrifugation step during DNA extraction. Cultivated species, both unnamed and named batches, according to HOMD classification are presented in A. Uncultured phylotypes are presented in B. Read-abundances were log transformed and all identified HOTs are presented here. Bubble sizes correspond to relative abundance values calculated from log-transformed 16S read-abundance data for each HOT. Standard deviation in HOT abundance between wells from the same batch ranged between 0.1% and 3% whereas standard deviation was higher (0.3 to 8%) between batches.