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Table 1 Quantitative PCR measures of fold changes in the total bacterial and in H. hepaticus loads from genomic DNA extracted from cecum

From: The effects of intestinal microbial community structure on disease manifestation in IL-10-/- mice infected with Helicobacter hepaticus

Treatment

Fold change ± SD

 

H. hepaticus infected

Total bacterial load

H. hepaticus load

Cefoperazone (cef) treatment

   

Control 1 (no cef)

No

calibrator

N/A

Control 2 (1 day recovery from cef)

No

-3,333.00 ± 0.34

N/A

Control 3 (6 wk recovery from cef)

No

2.16 ± 1.18

N/A

Community 1 (no cef)

Yes

1.25 ± 0.75

calibrator

Community 2 (1 day recovery from cef before infection)

Yes

4.48 ± 2.55

-1.12 ± 2.79

Community 3 (6 wk recovery from cef before infection)

Yes

-1.35 ± 0.71

3.94 ± 6.30

Vancomycin (vanc) treatment

   

Control 4 (no vanc)

No

1.17 ± 0.68

N/A

Control 5 (vanc)

No

1.95 ± 1.41

N/A

Control 6 (vanc + 11 days recovery)

No

1.41 ± 0.91

N/A

Community 4 (no vanc)

Yes

1.90 ± 1.91

1.04 ± 0.30

Community 5 (vanc)

Yes

1.98 ± 1.62

-1.59 ± 0.52

Community 6 (vanc + 11 days recovery)

Yes

1.14 ± 1.16

1.28 ± 0.56

Germfree mice infected with H. hepaticus

N/A

-2.94 ± 1.90

  1. The fold change reported (2 -ΔΔCt) is normalized to TNFα and is in comparison to the samples indicated as calibrator in the respective columns. N/A, not applicable.