Fig. 2

Card9^−/− mice susceptibility to colitis is not overridden by colonization with a WT microbiota. A Schematic representation of the DSS-induced colitis experiment preceded by 3 weeks of oral administration (gavage) of GF WT and GF Card9^−/− mice with the microbiota of WT mice (WT ➔ GF WT and Card9^−/− ➔ GF WT) or Card9^−/− mice (WT ➔ GF Card9^−/− and Card9^−/− ➔ GF Card9^−/−). DSS 2% in drinking water for 7 days and then water for 5 days. B Weight of DSS-exposed GF Card9^−/− or GF WT mice colonized with the microbiota of WT mice (WT ➔ GF Card9^−/− and WT ➔ GF WT) or Card9^−/− mice (Card9^−/− ➔ GF WT and Card9^−/− ➔ GF Card9^−/−). C Representative H&E-stained images of colon cross sections from DSS-exposed GF Card9^−/− colonized with the microbiota of WT mice (WT ➔ GF Card9^−/−, left) or Card9^−/− mice (Card9^−/− ➔ GF Card9^−/−, right) at day 12. Scale bars, 500 μm. D Histological score of colon sections at day 12. E Il-22, Reg3β, and Reg3γ expression by qRT-PCR in total colon tissue at day 12, normalized to Gapdh. IL-17, IL-6, IL-10, and IL-22 concentration measured by ELISA in total colon tissue (F) or mesenteric lymph nodes (MLN, G) of WT ➔ GF Card9^−/− and Card9^−/− ➔ GF Card9^−/.⁻ mice at day 12. Data points represent individual mice. Data are mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, as determined by two-way analysis of variance (ANOVA) with Sidak’s posttest (B) and Mann–Whitney test (D, E, F, G)