Fig. 4

Immune features of the small intestine lamina propria in IG and PW colonized mice at 8 weeks of age. A Rorγt + cells in the small intestine lamina propria shown as a percentage of CD4 + TCRβ + live cells. B Rorγt + Regulatory T cells (gated as Rorγt + FoxP3 + CD4 + TCRβ + live cells) in the small intestine lamina propria shown as a percentage of CD4 + TCRβ + live cells. C Macrophages (gated as F4/80 + CD11b + CD45 + live cells) in the small intestine lamina propria shown as a percentage of live cells. D IgA + Plasma Cells (gated as Lin- [CD3-TCRβ-CD4-CD11c-NK1.1-F4/80-] IgA + B220- live cells) in the small intestine lamina propria shown as a percentage of live cells. E Representative flow plots of IgA + Plasma Cells (gated on live Lin- cells) in HD and SD IG groups. F Levels of CCL5 and IL-1β in ileal tissue lysates from IG mice as measured by ELISA. G Levels of CCL5 and IL-1β in ileal tissue lysates from PW mice as measured by ELISA. Data shown pooled between healthy donor colonized mice (3176 and 4142) and stunted donor colonized mice (4092 and 3114). Each point represents an individual animal. A-D n = 20–24/group [10–12 per donor] for IG groups, n = 14–17/group [5–9 per donor] for PW groups. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 by Two-Way ANOVA with Šídák’s multiple comparisons test. F n = 18–20/group [6–13 per donor], * p ≤ 0.05 by Mann–Whitney U test