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Table 2 Multi-omic investigation of polymer degradation within the plastisphere. Annotations and relative abundances are based on the number of aligned metagenomic reads, or relative quantification of proteins, as a proportion of the total identified using PlasticDB as a reference

From: Novel functional insights into the microbiome inhabiting marine plastic debris: critical considerations to counteract the challenges of thin biofilms using multi-omics and comparative metaproteomics

Metagenome

PlasticDB enzyme

Substrate

PlasticDB species

PlasticDB phyla

Average ID (%)

Rel. ab. (%)

Laccase

PE

Psychrobacter sp.

Proteobacteria

80.3

36.4

Depolymerase

PLA

Marinobacter sp.

Proteobacteria

79.8

18.2

Dehydrogenase

PHA, P3HV/PHBV

Paracoccus denitrificans

Proteobacteria

80.8

18.2

Esterase

PLA

Uncultured bacterium

Uncultured bacterium

83.3

18.2

Depolymerase

PLA

Pseudomonas putida

Proteobacteria

78.7

9.1

Metaproteome

PlasticDB Enzyme

Substrate

PlasticDB taxonomy

Protein

Consensus taxonomy

Rel. ab. (%)

Depolymerase

PLA

Paucimonas lemoignei

Poly(3-hydroxyalkanoate) depolymerase C

Paucimonas lemoignei

12.4

Hydrolase

Nylon

Paenarthrobacter ureafaciens

6-aminohexanoate-dimer hydrolase

Paenarthrobacter sp.

28.3

Polyamidase

Nylon

Nocardia farcinica

6-aminohexanoate-cyclic-dimer hydrolase

Nocardia sp.

30.9

  1. PLA Poly lactic acid, PE Polyethylene, PHA Polyhydroxyalkanoate, PHB Polyhydroxybutyrate, P3HV/PHBV Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)