Fig. 5

Visualization Results of Untargeted Metabolomics Sequencing and Analysis. A, annotation results of the detected metabolites in the KEGG database; B-D, volcano plots of the significantly up-regulated (orange) and down-regulated metabolites (green) between the AMW and Control groups on days -3 (B), 30 (C), and 60 (D), respectively; E-G, bubble plots of the top 20 enriched KEGG pathways from the differentially expressed metabolites between the AMW and Control groups on days -3 (E), 30 (F), and 60 (G), respectively. In A, the colored terms on the left represent the primary classification of these pathways with the same color, while the numbers on the right side of the column indicate the count of metabolites that belong to each signaling pathway. In E-G, “Number” indicates the number of DE Metas which were annotated to the corresponding pathway: a bigger value indicates more DE Metas enriched in this pathway; “Ratio” indicates the ratio of the number of DE Metas which were enriched in the corresponding pathway to the number of annotated metabolites in this pathway: a bigger value indicates a higher enrichment level of DE Metas in this pathway; and “p-values” indicates the significance calculated by hypergeometric inspection. The color of symbols indicates their corresponding p-value: a smaller value indicates greater inspection reliability. VIP, variable importance in the projection; DE Meta, differentially expressed metabolites