Fig. 4

Comparison of the Serum Indicators Associated with the Obtained Enriched KEGG Pathways from the Transcriptomic Sequencing. A, results of the Principal Component Analysis (PCA) of gene expression data from calves’ blood based on fragments per kilobase million; B-D, ratio of lymphocyte counts to white cell counts (Lymphocyte Ratio), neutrophil counts, and basophil counts in the blood of calves in the AMW and Control groups at each time point; E-R, concentrations of immunoglobulin G, immunoglobulin M, immunoglobulin A, nitric oxide, interleukin (IL)-6, IL-8, interferon-γ, tumor necrosis factor-α, malondialdehyde, C-reactive protein, serum amyloid A, IL-2, IL-10, and heat shock 70 kDa protein in the blood of calves in the AMW and Control groups at each time point; S-X, activities of total antioxidant capacity, superoxide dismutase, nitric oxide synthase, hydroxyl radical, glutathione peroxidase, and catalase in the blood of calves in the AMW and Control groups at each time point. Data are expressed as the mean ± standard error mean (SEM). In A, the permutational multivariate analysis of variance was used to calculate the difference between the AMW and Control groups at each time point: ns, p > 0.05. In B, the Mann-Whitney U test was used to calculate the difference between the AMW and Control groups at each time point: ns, p > 0.05; *, p < 0.05. In C-X, the least significance difference method in the one-way analysis of variance was used to analyze the differences between the AMW and Control groups at each time point: ns, p > 0.05; *, p < 0.05; **, p < 0.01