Fig. 2

The Structure and Composition of the Nasopharyngeal Microbiota in Calves. A and B, the Chao1 and Shannon indexes of the nasopharyngeal microflora in calves; C, the results of the Unweighted Unifrac-based Principal Coordinates Analysis (PCoA) of nasopharyngeal microflora in calves; and D-K, the relative abundance of phyla (D, G, and J) and genera (E, H, and K) with > 1% relative abundance, and the genera with the top 10 contributions to the difference between the two groups (F, I, and L), in the nasopharyngeal microbiota on days -3, 30, and 60, respectively. The data are expressed as the mean ± standard error mean (SEM). In A and B, the least significance difference method in the one-way analysis of variance was used to analyze the differences between the AMW and Control groups at each time point: ns, p > 0.05; *, p < 0.05. In C, the permutational multivariate analysis of variance was used to calculate the difference between the AMW and Control groups at each time point: ns, p > 0.05; *, p < 0.05. In D-K, the Wilcox Rank-Sum test was used to analyze the difference in microbiota between the two groups at each time point: ns, p > 0.05; *, p < 0.05; **, p < 0.01