Fig. 3

Gut microbes mediate the protective effect of PEDV in Min pigs. A FMT model in Min pigs and Landrace pigs (NC, Landrace pigs without FMT treatment, n=5; L donor, Landrace pigs treated with FMT from Landrace pigs, n=8; M donor, Landrace pigs treated with FMT from Min pigs, n=8). Comparative analysis of survival (D), weight loss (B), and diarrhea (C) in each group of piglets. (G) Comparison of histopathological changes (H&E staining) and PEDV viral load in the jejunum of piglets on day 8 post-infection. The left image is scanned at a magnification of 40×, and the right image is scanned at a magnification of 200×. E Analysis of average fluorescence intensity. F Histopathological scoring of tissues. The results are presented as the means ± SD, and statistical significance was calculated by t tests for two groups and one-way ANOVA for four groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001