Fig. 5

Abundance of gene functions involved in involved acetogenesis, hydrogenases and sulfur cycling pathways in metagenomic and metatranscriptomic libraries and MAGs (a) Relative abundance of genes and transcripts for acetogenesis, hydrogen sensing/evolution/bifurcation (hydrogenases) and sulfur cycling in the metagenomic (MG) and metatranscriptomic (MT) contigs from the hindguts of E. Pulchripes and G. connexa. Acetogenesis includes heterotrophic acetogenesis via a combination of glycolysis, pyruvate:ferredoxin oxidoreductase (porA), Phosphotransacetylase (pta) and acetate kinase (ack), and reductive acetogenesis via the Wood–Ljungdahl pathway (WLP). Full names of the gene families and their corresponding KEGG IDs are available in Table S13 (b) A heatmap showing the abundance of genes and transcripts in each MAG with at least four acetogenic genes or one of the sulfate-reduction genes. The tree was reconstructed using 39 concatenated bacterial single copy gene (ribosomal proteins) from the MAGs