Fig. 5

Phylogenetic tree of (a) MT1 methyltransferase and (b) corrinoid proteins including sequences from metatranscriptomics and MAGs recovered from the metagenomics datasets. The evolutionary history was inferred using the neighbour-joining method. The optimal trees with the sum of branch length of 11.7 and 11.1 are shown for methyltransferase and corrinoid proteins, respectively. Bootstrap values (100 replicates) are shown as black (> 50) and grey (< 50%) dots. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All ambiguous positions were removed for each sequence pair. There was a total of 637 positions for methyltransferases and 217 positions for the corrinoid proteins in the final dataset