Fig. 3

Maximum likelihood phylogenetic tree of the mcrA gene from the cultured methanogens. The tree also contains the mcrA sequences (marked in bold) from the metagenomes and the MAGs obtained within this study. ModelFinder was used to find the best-fit model for the data [52]. Bootstrap values (1000 replicates) were shown as black dots (> 80%) and grey dots (< 80%). The tree is drawn to scale, with branch lengths accounting for substitutions per site. The genus Methanopyrales was used as the outgroup