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Fig. 4 | Microbiome

Fig. 4

From: Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity

Fig. 4

Effects of antibiotics on OM commensal bacteria and immunity. a The culture plates from trout OM (from left to right: Cor, BC, and FC) of control fish (upper) and 4-day treated fish with antibiotics (lower), respectively. Red triangles indicate a single colony of bacteria. b Numbers of bacterial colony in control and 4-day treated fish counted from a (n = 9). c The expression of V3-V4 16S rRNA region in the Cor, BC, and FC after treatment with antibiotics. d The expression of immune-related genes in the trout Cor, BC, and FC after treatment with antibiotics. AB, antibiotics. Statistical differences were evaluated by unpaired Student’s t test. ***p < 0.001

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