Fig. 2

Distribution characteristics of commensal microbiota in the Cor, BC, and FC of trout OM. a Representative dot plots showing the staining of bacteria on the Cor, BC, and FC with SYTO BC Green. b Microbial counts on the Cor, BC, and FC (n = 9). c, d Richness (c) and diversity (d) of bacterial community in Cor, BC, and FC of control trout OM (n = 4). Community richness and diversity were measured by Chao1 index and Shannon index, respectively. e Principal coordinate analysis (PCoA) with weighted UniFrac distance matrix for the Cor, BC, and FC microbiota community from control trout OM (n = 4). f Venn diagram showing the number of the same and different bacteria in the Cor, BC, and FC of control trout OM. g Violin plot showing the representative abundance of aerobic-, anaerobic-, facultatively anaerobic-, gram-negative-, gram-positive-, forms biofilms-, and potentially pathogenic bacteria in the Cor, BC, and FC from control trout OM (n = 4). One-way ANOVA test was used to evaluate the statistical differences. Data are presented as mean ± SEM of three biological duplicates. *p < 0.05, **p < 0.01, ***p < 0.001