Fig. 3

Alone-housed Phb1^ΔPC mice manifest Paneth cell defects and spontaneous ileitis. A H&E staining showing Paneth cells (pink granules) in the ileal crypts. Bar = 150 μm; boxed pullout bar = 75 μm. B Immunofluorescent-staining for lysozyme (red), muc2 (green), and DAPI (nucleus, blue) in ileal crypts (dashed line). Star denotes lysozyme/muc2 colocalization in the crypt base. Bar = 150 μm; boxed pullout bar = 75 μm. C Average number of lysozyme⁺ cells per crypt per mouse. A minimum of 50 crypts per mouse were quantitated. D Paneth cell lysozyme allocation patterns. A minimum of 50 crypts per mouse were quantitated. E mRNA quantification in the ileum. One outlier was identified by ROUT test (Q = 1%) and was removed from Phb1^ΔPC lysozyme, cryptdin5, and ang4). F AB-PAS staining of the ileum. Bar = 100 µm. The dashed line denotes the crypt base. G The number of AB-PAS⁺ cells/crypt base across 50 crypts. H Histological inflammation scoring of the ileum. The arrow denotes immune cell infiltration, and the arrowhead denotes villus blunting. Bar = 100 µm. I Histological inflammation scoring of H&E-stained ileal sections. J mRNA quantification in the ileum. n = 7 Phb1^fl/fl or 11 Phb1^Δ.^PC mice. AB-PAS, alcian blue-periodic acid Schiff; Ang4, angiogenin 4; H&E, hematoxylin & eosin; Ifng, interferon gamma; Il1b, interleukin 1 beta; muc2, mucin2; Tnfa, tumor necrosis factor alpha. Results are presented as individual mice ± SD. *P < 0.05, **P < 0.01, and ****P < 0.0001