Fig. 8

ARG assembly from metagenomic data improved by GLOPS and ADAG algorithms. A Reduction of the total number of seed segments after GLOPS. B Increase in seed segment length and k-mer count after GLOPS. C Path optimization using ADAG resulting in the reduction of the total number of segments/links in the GFA, with an increase in segment length and k-mer count. Note that all density plots have a log-scale x-axis. Sequence homology search using BLASTn on the segments constituting the final GFA paths returned at least one match with coverage of 97% (± 9%) and identity of 99.7% (± 1%) for 92% segments (818 ± 475 nt). This indicates that the GFA paths generated and filtered by the implemented steps represent real, biologically meaningful sequences