Fig. 5

Bacterial infections using different culture conditions. A Colony forming unit (CFU) analysis and B relative growth of the S. aureus clinical isolate SA-DUS-011 after 24 h of colonization applying four different methods (glass cylinder methodology (25 µL) versus small droplet (5 µL) and 37 °C (humid) versus 32 °C (dry)) (N = 3 per method) (input at 0 h), *p < 0.05 (Mann-Whitney U test, CFU outcome of 37 °C glass cylinder method compared to the other methods). C Percentage of infected HEEs (N = 5 per method), cultured and exposed for up to 10 days with SA-DUS-011 applied using the four different methods