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Fig. 2 | Microbiome

Fig. 2

From: Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

Fig. 2

Colonization of HEEs with skin pathogens and commensals. A Growth and viability analysis by means of colony forming unit (CFU) count (input at 0 h) (biological N = 4) and B gene expression analysis of the antimicrobial peptides DEFB4 (gene encoding hBD2), S100A9 (MRP14) and PI3 (SKALP/elafin) after 24 h of exposure to two S. aureus strains (ATCC 29213 and the clinical isolate SA-DUS-011) (biological N = 4, all controls set at 1). C CFU count (input at 0 h) (N = 3) and D gene analysis of DEFB4, S100A9, and PI3 after 24 h of culture with skin related bacteria (S. epidermidis = Se, C. acnes = Ca, C. aurimucosum = Cau, S. capitis = Sc) (N = 3, control set at 1 (dashed line)). *p < 0.05, **p < 0.01. Mean ± SEM

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