Fig. 2
Precision and recall in genome reconstruction with single-cell-guided binning of metagenome assembly. The characteristics of metagenome bins collected using SMAGLinker and conventional 4 metagenome binners (DAS_tool, MetaBAT 2, MAXbin 2, and CONCOCT) were evaluated using the same dataset obtained from a mock microbial community containing 15 bacterial species. (a) Total metagenome assembly (MA) lengths of the contigs incorrectly assigned to different bacterial bins, namely “incorrectly binned contig”, and contigs unbinned to any reference genomes, namely “unbinned contig”. (b) Correlation between the completeness of non-redundant single-cell amplified genomes (nrSAGs) and total length of unbinned contigs against the target single-cell genome-guided bin (sgBin) in SMAGLinker. B. pseudocatenulatum, C. acnes, and C. terrigena had no unbinned contigs. (c) The plots of F1 scores, precision, and recall of all reported bins in SMAGLinker and four conventional metagenome binners (center line, median; box limits, upper and lower quartiles; whiskers, minimum or maximum values between upper and lower quartiles, which are extended 1.5 times the interquartile region). Individual values are represented as dots