Effect of postnatal low-dose exposure to environmental chemicals on the gut microbiome in a rodent model

Microbiome

Table 2 Experimental plan of environmental chemicals treatment and stool sampling

Group	Compound (dose in mg/kg bw)^a	Animals at start	Monitored animals
Group	Compound (dose in mg/kg bw)^a	No.	PND 62	PND 181
I	DEP (0.1735)	20	20	10
II	MPB (0.1050)	20	20	10
III	TCS (0.050)	20	20	10
IV	MIX (mixture of DEP + MPB + TCS)	20	20	10
V	OIL	20	20	10
Total		100	100	50

DEP diethyl phthalate, CAS 84-66-2, MPB methylparaben, CAS 99-76-3, TCS triclosan, CAS 3380-34-5), MIX mixture of DEP + MPB + TCS in equal quantities, OIL olive oil control, vehicle alone
^aAnimals were treated from PND 1 to PND 181. The newborns (F1) were first exposed to chemicals postnatally through milk from the exposed dams, from birth to weaning (PND 28). After weaning, these pups were exposed through oral gavage three times a week, from PND 28 to PND 181. Each compound was administered in olive oil as vehicle by gastric intubation (gavage), starting with 0.5 ml of olive oil from 4 to 9 weeks of age, and then with 1 ml once adult

ISSN: 2049-2618