An in vitrobiofilm model system maintaining a highly reproducible species and metabolic diversity approaching that of the human oral microbiome

Table 2 Numbers of human oral taxa (HOT) within the most dominant genera in saliva and biofilm samples

Bacterial phyla (bold) and genera (italics)	Number of HOT identified	Number of HOT identified *in vitro*	Number uncultivated		Number uncultivated
	Number of HOT identified	Number of HOT identified *in vitro*	Phylotypes (P)		Phylotypes (P)
	Saliva^a	Biofilm^a	HOMD^a		HOMD^b
	Saliva^a	Biofilm^a	Saliva	Biofilm	Saliva	Biofilm
Firmicutes
Streptococcus	25 (58)	38 (58)	6	11	25	25
Veillonella	3 (9)	2 (9)	1	1	5	5
Parvimonas	4 (6)	0 (2)	1	ND	1	1
Mogibacterium	4 (5)	0 (5)	ND	ND	ND	ND
Gemella	3 (3)	3 (3)	ND	ND	ND	ND
Peptostreptococcus	3 (3)	0 (3)	3	ND	ND	ND
Granulicatella	1 (2)	1(2)	ND	ND	2	2
Klebsiella	0 (2)	2 (2)	ND	ND	ND	ND
Lactobacillus	0 (0)	1(2)	ND	ND	ND	1
Bacteroidetes
Prevotella	3 (38)	0 (11)	1	ND	3	3
Porphyromonas	1 (8)	0 (6)	ND	ND	4	4
Fusobacteria
Fusobacterium	4 (12)	2 (11)	2	ND	5	5
Proteobacteria
Neisseria	5 (11)	0 (7)	ND	ND	ND	ND
Campylobacter	1 (5)	0 (3)	1	ND	ND	ND
TM7:
TM7 (G-1)	0 (2)	0 (0)	ND	ND	2	ND
TM7 (G-2)	0 (0)	0 (1)	ND	ND	ND	1
TM7 (G-3)	0 (1)	0 (0)	ND	ND	1	ND

HOT designations were defined according to the Human Oral Microbiome Database (HOMD) classification scheme. (), Total numbers of HOT, including singletons, are presented inside brackets. ^aUncultivated phylotypes were identified based on the HOMD classification. The numbers of phylotypes representing HOT designations with >200 16S gene counts (corresponding to >0.3% of total 16S counts) are presented in these columns. ^bAll HOT designations (including those that were represented by 1 to 200 copies of16S gene reads). ND, no uncultivated HOMD representative was detected.

ISSN: 2049-2618